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Antibodies bound to the blocking peptide now not bind to the epitope within the focus on protein. By comparing the staining within the blocked antibody compared to the antibody by itself, you can see which staining is certain.
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This manual outlines further attainable optimization of mobile and immunoassay parameters to make certain the very best outcomes are...
This tutorial outlines additional doable optimization of cellular and immunoassay parameters to be certain the best possible benefits are...
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The antibody is from purified rabbit serum by affinity purification by using sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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The measurement of protein phosphorylation is a useful tool for measuring the modulation of receptor activation by get more info both equally antibodies...
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Blocking peptides are peptides that bind specifically towards the goal antibody and block antibody binding. These peptide generally incorporates the epitope recognized via the antibody.
Focus on Information and facts Cyclin E belongs to your remarkably conserved cyclin spouse and children, whose members are characterized by a remarkable periodicity in protein abundance through the mobile cycle. Cyclins perform as regulators of CDK kinases. Various cyclins show unique expression and degradation patterns which contribute to the temporal coordination of each mitotic party. Cyclin E sorts a complex with and functions as a regulatory subunit of CDK2, whose activity is required for mobile cycle G1/S changeover. This protein accumulates within the G1-S stage boundary which is degraded as cells progress through S period.
Overexpression of this gene has been noticed in many tumors, which results in chromosome instability, and thus might add to tumorigenesis. This protein was found to affiliate with, and become linked to, the phosphorylation of NPAT protein (nuclear protein mapped to the ATM locus), which participates in mobile-cycle regulated histone gene expression and performs a crucial function in endorsing mobile-cycle development during the absence of pRB. Two alternatively spliced transcript variants of the gene, which encode unique isoforms, are already explained. Two further splice variants ended up reported but comprehensive nucleotide sequence data is not really but available.
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